Fig 2. Metabolic activity of C. auris cells in biofilm decreases with increasing maturity.

C. auris strain MRL 5765 cells were stained with FUN-1 and ConA- Alexa Fluor 488 conjugate stain after incubation of biofilm for 4h, 12h and 24h respectively. The image utilizes a 63X oil immersion objective with magnification, X1. The cells that were live and active produce orange-red cylindrical intra-vacuolar structures (CIVS). The increase in green fluorescence shows the increase in biofilm extracellular matrix with increasing incubation time.