Fig 2. Model convergence to steady state.

(A,B) The single-cell O’Hara-Rudy model was paced at 1Hz frequency for 1000 s, from different initial intracellular concentrations (initial concentrations are listed on panel A). AP waveform on the 1^st, 10^th and 1000^th beats are depicted by dotted, dashed and solid lines correspondingly. (C, D) [Na⁺]_i and [Ca²⁺]_nsr concentrations change during the simulations. (E, F) Box-and-whiskers plots depict GA output [Na⁺]_i and [Ca²⁺]_nsr concentrations distance from steady state values.