Fig 4. ArdC DNA-binding preferences assessed by EMSA.

ArdC binding of a 6FAM-labeled 45 bases ssDNA oligonucleotide (Fluor-T87I2), a perfectly paired complementary 45bp dsDNA duplex (Fluor-T87I2 + T87I1), and two partial dsDNA with 5’ or 3’ terminal ssDNA overhangs (Fluor-T87I2 + Mid1) and (Fluor-T87I2 + Mid2) was performed at increasing concentrations of ArdC (0, 125 nM, 250 nM, 500 nM, and 1 μM), as described in Materials and Methods. Protein-DNA complexes were resolved by native 10% polyacrylamide gels and visualized by a fluorescent image analyzer.