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. 2020 Apr 29;16(4):e1008750. doi: 10.1371/journal.pgen.1008750

Fig 5. Effect of ArdC E229A mutant on plasmid conjugative transfer.

Fig 5

A) Conjugation of E. coli BW27783 bearing pSU2007 or pLGM33 (pSU2007_ardC_E229A) to P. putida KT2440. Conjugation was performed for 1 h at 37°C. Horizontal bars represent the mean ± SD of N = 3 observations. B) Effect in the conjugation frequency of pLGM25 when expressing ardC_E229A in recipient cells. Conjugation of pLGM25 in E. coli BW27783 donor cells to P. putida KT2440 recipient cells bearing pUCP22::ardC or pUCP22:: ardC_E229A. Conjugation was performed for 1 h at 37°C with 0.1 mM IPTG added to the mating mixture (N = 9).