Skip to main content
. 2020 Apr 29;16(4):e1008750. doi: 10.1371/journal.pgen.1008750

Fig 6. Effect of HsdRMS systems on R388 plasmid conjugation.

Fig 6

A) Effect of ArdC on plasmid conjugative transfer from E. coli to P. putida KT2440 mutants. The conjugation frequencies (T/R) to P. putida KT2440 wt strain or different mutants were obtained after conjugation for 1h at 37°C. The deleted gene(s) in each strain is shown. EM42 is Δprophage1, Δprophage4, Δprophage3, Δprophage2, Δtn7, ΔendA-1, ΔendA-2, ΔhsdRMS, Δflagellum, and Δtn4652. ΔP1-P4 stands for Δprophage1 Δprophage4 Δprophage3 Δprophage2 strain. Horizontal bars represent the mean ± SD obtained for each dataset of N = 8–12 (Student's t-test: * p < 0.1,** p < 0.01, *** p < 0.001, **** p <0.0001). The effect of ardC in the transfer of T-DNA methylated or not to restricting or non-restricting P. putida B) or E. coli recipients C) is evaluated in mating experiments performed as in A with N = 6.