Table 2. Strains used in this study.
Strain | Phenotype | Reference |
---|---|---|
Escherichia coli | ||
DH5α | F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG, Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK- mK+), λ– | [33] |
BL21 (DE3) | F– ompT gal dcm lon hsdSB(rB–mB–) λ(DE3[lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) | [34] |
BL21(DE3)-SmR | Streptomycin resistant spontaneous mutant of BL21(DE3) | This work |
C41 (DE3) | F- ompT hsdSB (rB- mB-) gal dcm (DE3) | [35] |
β834(DE3) | F- ompT hsdSB(rB- mB-) gal dcm met (DE3) | [36] |
TB10 | TB10 is the result of a P1 transduction from DY329 into MG1655. It has a large amount of the λ prophage genome inserted into a biotin operon. The λ red genes α, β and γ are under the control of cI857, making it temperature inducible. | [37] and [38] |
DY380 | SmR λ Cl857 (cro-bioA) tet (DH10B) | [39] |
BW27783 | F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514 Δ(araH-araF)570(::FRT), ΔaraEp-532::FRT, φPcp8araE535 | [40] |
BW27783-NxR | Nalidixic resistant spontaneous mutant of BW27783 | [41] |
BW27783-RifR | Rifampicin resistant spontaneous mutant of BW27783 | [41] |
MG1655 | K-12 F–λ–ilvG–rfb-50 rph-1 | [42] |
MG1655-RifR | Rifampicin resistant spontaneous mutant of MG1655 | This work |
EcMR2ΔmutS | MG1655, lacI- bla, bio-, lambda-Red1, mutS–, cmR | [43] |
Pseudomonas putida | ||
KT2440 | Wild-type P. putida strain; mt-2 derivative cured of its plasmid (pWW0-) | [44] |
EM178 | KT2440 derivative; Δprophage1 Δprophage4 Δprophage3 Δprophage2 | [45] |
EM42 |
KT2440 derivative; Δprophage1 Δprophage4 Δprophage3 Δprophage2 Δtn7 ΔendA-1 ΔendA-2 ΔhsdRMS Δflagellum Δtn4652 | [45] |
EM422 | KT2440 derivative; ΔhsdRMS | From De Lorenzo group |
KT2440 ΔrecA | KT2440 derivative; ΔrecA | From De Lorenzo group |
KT2440 Δflagellum | KT2440 derivative; Δflagellum | From De Lorenzo group |
KT2440 ΔendA1 | KT2440 derivative; ΔendA-1 | From De Lorenzo group |
KT2440 ΔendA2 | KT2440 derivative; ΔendA-2 | From De Lorenzo group |
KT2440 Δtn7 | KT2440 derivative; Δtn7 | From De Lorenzo group |
KT2440 Δtn4652 pSW | KT2440 derivative; Δtn4652 bearing pSW plasmid | From De Lorenzo group |
KT2440 Δtn4652 | KT2440 Δtn4652 derivative cured of pSW plasmid | This work |
Other bacteria | ||
Salmonella typhimurium LT2 |
Salmonella enterica subsp. enterica serovar typhimurium str. LT2 |
ATCC 700720 |
Klebsiella pneumoniae K6 |
K. pneumoniae subsp. pneumoniae. Clinical isolate from the Medical College of Virginia, 1994. ApR KmR, CmR | ATCC 700603 |
Acinetobacter baumannii |
SmR ApR | ATCC 19606 |
Vibrio cholerae CIP106855 |
N16961 RifR. Biovar Eltor, serovar O:1 | CIP106855 |
Agrobacterium tumefaciens GMI9023 | Agrobacterium tumefaciens C58 derivative cured of its plasmids (pTi-, pAT-) | [46] |