Table 3. Plasmids used in this study.
| Plasmid | Description | Phenotype | Size (Kb) | Reference |
|---|---|---|---|---|
| R388 | R388 wild type plasmid | SuR TpR; (IncW) | 33.9 | [47] |
| pSU2007 | R388 derivative; KnR cassette insertion | SuR TpR KnR; (IncW) | 32.9 | [48] |
| pET29c | Expression vector | KnR; Rep (pMB1); Overexpression controlled by T7 promoter with a 6-HisTag. |
5.4 | Addgene |
| pUA66 | GFP reporter plasmid | KnR pSC101 replicon | 4.5 | [49] |
| pUCP22 | Shuttle Vector; Escherichia-Pseudomonas broad-host-range expression vector | ApR GmR; Plac promoter. |
4.7 | [50] |
| pHERD20T | Shuttle Vector; Escherichia-Pseudomonas broad-host-range expression vector | CbR; PBAD promoter and araC regulator. | 5.1 | [51] |
| pLGM21 | pET29c::ardC | KnR T7 promoter | 6.1 | This work |
| pIC10 | R388ΔkfrA-orf14 | TpR KnR | 26.2 | This work |
| pLGM25 | R388ΔardC | TpR KnR | 33.9 | This work |
| pLGM28 | pET29c::ardC_E229A | KnR T7 promoter | 6.1 | This work |
| pLGM33 | pSU2007 (ardC_E229A) | TpR KnR | 32.9 | This work |
| pLGM36 | pUCP22::ardC | ApR GmR Plac promoter | 5.6 | This work |
| pLGM37 | pUCP22::ardC_E229A | ApR GmR Plac promoter | 5.6 | This work |