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. Author manuscript; available in PMC: 2020 May 11.
Published in final edited form as: Chem Biol Drug Des. 2011 Apr 27;77(6):421–430. doi: 10.1111/j.1747-0285.2011.01108.x

Figure 6. Immunofluorescence confocal microscopy of IC/PBS cell explants treated with d-pipecolic acid as-APF, d-proline as-APF, or vehicle (PBS) alone for 9 days.

Figure 6

Explanted cells from an IC/PBS donor were treated with 2.5 μM d-proline as-APF, d-pipecolic acid as-APF, or diluent (PBS) control twice weekly. On day 9, cells were fixed with acetone/ethanol and incubated with FITC-labeled anti-ZO-1 antibody, or primary antibodies against claudin or occludin proteins followed by FITC-labeled secondary antibodies. Images were obtained on a Zeiss LSM510 confocal laser scanning microscope. (Figure is representative of three separate experiments using cells from 3 IC/PBS donors)