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. Author manuscript; available in PMC: 2020 May 11.
Published in final edited form as: Chem Biol Drug Des. 2011 Apr 27;77(6):421–430. doi: 10.1111/j.1747-0285.2011.01108.x

Figure 8. Decreased paracellular permeability of IC/PBS cells by d-pipecolic acid as-APF.

Figure 8

Paracellular flux of [3H]-inulin (molecular weight: 5,200) or [14C]-mannitol (molecular weight: 184) was measured in IC/PBS cells cultured on Transwell membranes and treated with 2.5 μM d-pipecolic acid as-APF (□), inactive peptide control ( Inline graphic), diluent control (■), or calcium free medium ( Inline graphic) for 16 days prior to incubation with tracer. Data are expressed as mean percent of the radioactivity applied to the apical medium that was recovered in the basal medium at 0.5 hr, 1 hr, 2 hrs, 4 hrs, 6 hrs +/- standard deviation. (Data shown from 3 experiments using cells from 3 different IC/PBS donors).