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. Author manuscript; available in PMC: 2020 May 11.
Published in final edited form as: Cancer Res. 2009 Jan 20;69(3):758–764. doi: 10.1158/0008-5472.CAN-08-2984

Figure 3.

Figure 3

Representative COBRA assays for validation of array analyses. PCR primers for each of the eight CpG targets are given in Supplementary Table S4. One half of each PCR product was run on 3% agarose gel neat (−) and the other half after Taq1 restriction digest (+). AIM2, CARD15, CHI3L2, and EPHX1 show hypomethylation in FL samples relative to FH, indicated by diminished Taq1 cutting of PCR product. EPHA7, ETV1, PITX2, and TFAP2C show hypermethylation in FL relative to FH, indicated by greater Taq1 restriction digestion of FL-derived PCR products. These results are in accordance with array measures.