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. 2020 Mar 26;5(6):e134049. doi: 10.1172/jci.insight.134049

Figure 2. Lack of IAP is associated with a severe aging-related liver inflammation and an increased proinflammatory characteristic of portal serum.

Figure 2

(A and B) Liver Tnfa and Il6 mRNA levels measured by qPCR. (C) Liver macrosteatosis score (Macrosteatosis 0%–5%, grade 0; 5%–33%, grade 1; 33%–66%, grade 2; 66%–100%, grade 3). (D) Oil Red O staining of liver (magnification, 20×). (E) Portal serum endotoxin level measured by LAL assay. (F) Tnfa mRNA level of primary mouse BM-derived macrophages incubated with defined systemic or portal serum for 24 hours. Each group included 5 animals, and data are representative of 3 biological replicates. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, as measured by ANOVA with multiple-comparisons post hoc tests (Tukey’s HSD).