Figure 1. A distinct population of Tregs is identified in the synovial fluid of sJIA patients with chronic arthritis.

(A) viSNE plots of mass cytometry of live, single cells from 2 chronic sJIA patients with paired peripheral blood (PB) and synovial fluid (SF) samples. The color indicates the lymphocyte populations, which were manually gated and overlaid onto the viSNE plots. (B) Gated, live, single Tregs (CD4⁺CD25⁺CD127^lo) from the PB and SF of each chronic sJIA patient were analyzed together by viSNE and overlaid on the same viSNE plot. (C) viSNE plots of mass cytometry of PB and SF Tregs from 2 sJIA patients with paired PB and SF samples. The color indicates cell expression level of the labeled marker. viSNE analysis was performed with Cytobank. sJIA, systemic juvenile idiopathic arthritis; N, naive; M, memory; viSNE, visualization using t-Distributed Stochastic Neighbor Embedding; tSNE, t-Distributed Stochastic Neighbor Embedding.