Figure 6. The Th17 reprogramming of Tregs in sJIA is reversed in patients treated with first-line rIL-1RA.

(A) Representative flow cytometry dot plots gated on peripheral blood (PB) Tregs (CD4⁺CD25⁺FOXP3⁺). (B) The mean percentage ± SD of IL-17⁺ Tregs (CD4⁺CD25⁺FOXP3⁺) in acute sJIA patients (n = 15) compared with patients treated with first-line rIL-1RA (within 4 weeks of diagnosis) who entered remission (n = 7). Pediatric controls are also depicted (n = 5). (C) The mean percentage ± SD of IL-17⁺ Tregs (CD4⁺CD25⁺FOXP3⁺) in 4 sJIA patients at disease onset and after remission was induced by first-line treatment with rIL-1RA (1-way ANOVA corrected for multiple comparisons, **P ≤ 0.01). sJIA, systemic juvenile idiopathic arthritis; Tx, treatment; Unstim, unstimulated; PB, peripheral blood; Pedi, pediatric