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. 2020 Apr 28;8(1):e000544. doi: 10.1136/jitc-2020-000544

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© Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution 4.0 Unported (CC BY 4.0) license, which permits others to copy, redistribute, remix, transform and build upon this work for any purpose, provided the original work is properly cited, a link to the licence is given, and indication of whether changes were made. See https://creativecommons.org/licenses/by/4.0/.

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Multiparameter immunofluorescence histology of the vaccine site microenvironment postvaccination with peptides in IFA and pICLC showing co-expression of CD40L and CD3 but not CD8. Selected markers include Dapi (blue), CD40L (red), CD3 (yellow), CD8 (green). (A) Includes CD40L and (B) excludes CD40L. White arrows depict CD3⁺CD8^neg T cells co-expressing CD40L and CD3 but not CD8. Red arrows depict cells expressing CD40L but not CD3 or CD8.