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. 2020 May 7;13:3853–3866. doi: 10.2147/OTT.S232918

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© 2020 Liu and Wang.

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MiR-377-3p was negatively interacted with OIP5-AS1 in MG63/CDDP and Saos-2/CDDP cells. (A) The complementary sequences between OIP5-AS1 and miR-377-3p were exhibited, as well as the mutant sequences of OIP5-AS1. (B–C) The luciferase activity of OIP5-AS1-WT or OIP5-AS1-MUT reporter in MG63/CDDP and Saos-2/CDDP cells transfected with miR-NC or miR-377-3p was assessed by dual-luciferase reporter assay. (D and E) RIP assay was applied to further verify the interaction between OIP5-AS1 and miR-377-3p in MG63/CDDP and Saos-2/CDDP cells. (F and G) The level of miR-377-3p in CDDP-resistant tissues or cells was measure by qRT-PCR. (H) The correlation between miR-377-3p and OIP5-AS1 was analyzed via Pearson test. (I) The level of miR-377-3p in MG63/CDDP and Saos-2/CDDP cells transfected with si-NC, si-OIP5-AS1, pcDNA-NC, or pcDNA-OIP5-AS1 was detected via qRT-PCR. *P<0.05.