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. 2020 May 7;13:3853–3866. doi: 10.2147/OTT.S232918

Figure 5.

Figure 5

FOSL2 was a candidate target of miR-377-3p in MG63/CDDP and Saos-2/CDDP cells. (A) The complementary base pairing between 3ʹUTR of FOSL2 and miR-377-3p were presented, as well as the mutant sequences of FOSL2 3ʹUTR. (B and C) The luciferase activity of FOSL2 3ʹUTR-WT or FOSL2 3ʹUTR -MUT reporter in MG63/CDDP and Saos-2/CDDP cells transfected with miR-NC or miR-377-3p was evaluated by dual-luciferase reporter assay. (D–G) The mRNA and protein levels of FOSL2 in CDDP-resistant OS tissues, cells or normal tissues, cells were examined via qRT-PCR and Western blot assay, respectively.(H) The correlation between miR-377-3p and FOSL2 was processed via Pearson test. (I and J) The mRNA and protein levels of FOSL2 in MG63/CDDP and Saos-2/CDDP cells transfected with miR-NC or miR-377-3p were detected via qRT-PCR and Western blot assay. *P<0.05.