Figure 4.
SRF231 does not induce RBC aggregation or RBC phagocytosis. (A) Human whole blood was stained with the indicated concentrations of SRF231 or isotype control (hIgG4) and analyzed by flow cytometry gating specifically on the RBC population. (B) Human whole blood was incubated with 100 µg/mL of the indicated antibody, overnight at 37°C, after which blood smears were performed on glass slides and analyzed by light microscopy. scale bar=100 µm. (C) Human RBCs were incubated with the indicated concentrations of antibody and evaluated by flow cytometry. Data are presented as the percent doublets gated on a side scatter-height versus side scatter-width plot. Data points for 2 individual donors are plotted. (D) Human RBCs were CFSE labeled and incubated with human macrophages in the phagocytosis assay, as described in the materials and methods. CFSE, carboxyfluorescein succinimidyl ester; hIgG4, human IgG4; RBC, red blood cell.