Figure 5.

Differential gene and protein expression in healthy donor (HD) NK and high affinity natural killer (haNK) cells in response to hypoxia. HD NK and haNK cells were incubated in 20% or 0% oxygen for 5 hours at 37°C. RNA from two HD NK and haNK cells was extracted and RNAseq was performed in triplicate to analyze changes in gene expression in response to hypoxia. (A) Number of genes (and per cent) changed by <20% or >20% in HD NK and haNK cells. (B) Changes in RNA expression of genes important to NK cell function are presented as the ratio of the gene RNA transcripts per million (TPM) of expression under 0% O₂ vs the 20% O₂ in log2 space. (C) Number of proteins assayed and detected for both HD NK and haNK samples. (D) Diagram of proteins exclusive to HD NK or haNK cells as well as selected proteins shared between both cell types. Quantification of selected proteins is shown in (E). Each sample was run in triplicate and results are presented as mean±%coefficients of variation.