Figure 5. Crn1 and AcrIII-1 bind cA₄ with high affinity.

Phosphorimages of native gel electrophoresis visualising radiolabelled cyclic oligoadenylate (cOA) binding by (A) Crn1 (B) and catalytically inactive AcrIII-1 (SIRV1 gp29 H47A). Crn1 binds cA₄ (10 nM) with an apparent dissociation constant (K_D) of ~50 nM, whereas AcrIII-1 binds cA₄ with an apparent K_D of ~25 nM. The images shown are representative of three technical replicates. Control c – cOA alone. The other bands near the bottom of the gel are caused by unreacted ATP and other linear products.