Figure 3. The DAF-1/TGFβ receptor functions redundantly in interneurons to regulate peroxide resistance in response to DAF-7/TGFβ from ASI.
(A–B) daf-3(mgDf90) almost completely abrogated the increased peroxide resistance of (A) daf-1(m40) and of (B) genetic ablation of ASI. (C) Diagram of the subsets of neurons where daf-1(+) or daf-3(+) were expressed in transgenic rescue experiments shown in panels (D–K) and in Figure 3—figure supplement 1. The promoter elements used to drive gene expression in those subsets of neurons are shown in parentheses. The tdc-1 promoter also drives gene expression in the sheath cells of the somatic gonad. (D–I) Peroxide resistance of transgenic nematodes expressing daf-1(+) in specific subsets of cells and daf-1(m40) controls. (J–K) Peroxide resistance of transgenic nematodes expressing daf-3(+) in specific subsets of cells and daf-1(m40); daf-3(mgDf90) controls. (L) ASI signals to three sets of interneurons to lower the nematode’s peroxide resistance. To increase peroxide resistance, all of these sets of neurons must independently activate the DAF-3/DAF-5 transcriptional complex. See also Figure 3—figure supplement 1. Statistical analyses are in Supplementary file 3.
Figure 3—figure supplement 1. The DAF-1/TGFβ receptor functions redundantly in interneurons to regulate peroxide resistance in response to DAF-7/TGFβ from ASI.
