Fig. 5.

Jackstraw analysis of Cell Hashing data with HTO Demux (Stoeckius et al., 2018). Eight donors provided PBMCs, which are pooled and sequenced together using Cell Hashing (Stoeckius et al., 2018). Identities of 16 916 single cells are estimated by applying the HTODemux algorithm in Seurat. HTO-A thru HTO-H are singlets, corresponding to eight donors. Independently, the proposed method is applied on HTOs, resulting in 16 916 P-values for individual cells. The jackstraw P-values are in strong agreement with HTODemux classifications