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. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: Biochim Biophys Acta Mol Cell Biol Lipids. 2020 Mar 12;1865(7):158681. doi: 10.1016/j.bbalip.2020.158681

Figure 9: Trans-Δ2-hexadecenal forms adducts with glutathione, and HDAC1 in vitro.

Figure 9:

(A) Incubation of Δ2-HDE with glutathione (GSH) in vitro resulted in formation of Michael adducts (1), and Schiff’s base adducts (2) as determined by LC-MS/MS. (B) Incubation of Δ2-HDE with recombinant hHDAC1 in vitro generated five different adducts (left panel) with the Schiff’s base (imine) of lysine and Δ2-HDE by far was the most predominant species generated as detected by LC-MS/MS. This observation is consistent with the high amount of Lys residues in the sequence of hHDAC1 (40x) and their exposed localization on the protein’s surface (shown in cyan, right panel).