KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 | Thermo Fisher Scientific | Cat#A-11001; RRID:AB_2534069 |
| Polyclonal serum to SFTSV NP (Anti-NP) | Wenhui Li (National Institute of Biological Sciences) | N/A |
| Monoclonal anti-HRTV NP (2AG8) | Amanda E. Calvert (U.S. Centers for Disease Control and Prevention) | N/A |
| Bacterial and Virus Strains | ||
| Severe Fever with Thrombocytopenia Syndrome virus (SFTSV) or Huaiyangshan banyangvirus | Brennan et al., 2015 | N/A |
| Heartland virus | BEI Resources | NR-49771 |
| Escherichia coli BL21(DE3) | Novagen | Cat#69450 |
| Biological Samples | ||
| Fetal Bovine serum (GIBCO) | Thermo Fisher Scientific | Cat#16140071 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| L-742,001 | Sigma-Aldrich | Cat#SML1010–5MG |
| Baloxavir acid | Probechem | Cat#PC-35417–5MG |
| Sypro Orange | Invitrogen | Cat#S6650 |
| TALON® Metal Affinity Resin | Takara | Cat#635504 |
| TransIT-LT1 | Mirus Bio | Cat#MIR2300 |
| Trypsin | Hampton Research | Cat#HR2–429-02 |
| Deposited Data | ||
| Structure of SFTSV L endonuclease domain | This paper | PDB: 6NTV |
| Experimental Models: Cell Lines | ||
| Vero E6 | ATCC | Cat#CRL-1586; RRID:CVCL_0574 |
| 293T | ATCC | Cat#CRL-3216; RRID:CVCL_0063 |
| Oligonucleotides | ||
| 5’-FAM-AGGAAGAUUAAUAAUUUUCCU-Iowa Black® FQ-3’ |
This paper | N/A |
| Recombinant DNA | ||
| pRev (T7 RNA polymerase expressing plasmid) | Julie Pfeiffer (The University of Texas Southwestern Medical Center) | N/A |
| pTM-L | Brennan et al., 2015 | N/A |
| pTM-N | Brennan et al., 2015 | N/A |
| pTVT-L | Brennan et al., 2015 | N/A |
| pTVT-M | Brennan et al., 2015 | N/A |
| pTVT-S | Brennan et al., 2015 | N/A |
| SFTSV L 1–231aa | This paper | N/A |
| HRTV L 1–231aa | This paper | N/A |
| IAV L 1–192aa | DuBois et al., 2012 | N/A |
| Software and Algorithms | ||
| HKL3000 | Minor et al., 2006 | https://hkl-xray.com/hkl-3000 |
| CCP4i | Winn et al., 2011 | https://www.ccp4.ac.uk/ccp4i_main.php |
| Phenix | Adams et al., 2010 | https://www.phenix-online.org/ |
| WinCoot | Emsley et al., 2010 | http://bernhardcl.github.io/coot/ |
| Pymol 2.3 | Schrodinger | https://pymol.org/2/ |
| ASTRA 6 | Wyatt | https://www.wyatt.com/products/software/astra.html |
| Origin 7 | OriginLab | https://www.originlab.com/ |
| Prism 7 | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| MagTran | Zhang and Marshall, 1998 | N/A |
| Byonic and Byologic | Protein Metrics | https://www.proteinmetrics.com/ |
| HDExaminer | Sierra Analytics | http://massspec.com/hdexaminer/ |
| Gromacs 5.1.1 | Abraham et al., 2015 | http://manual.gromacs.org/5.1.1/index.html |
| FAST algorithm | Zimmerman and Bowman, 2015, 2016 | N/A |
| Surflex-dock | Jain, 2007 | https://omictools.com/surflex-dock-tool |