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. 2020 Apr 24;61(5):359–370. doi: 10.3349/ymj.2020.61.5.359

Fig. 5. Effects of restoration of miR-342-3p in osteosarcoma (OS) cell lines. Restoration of miR-342-3p in U2OS and MG-63 cells was induced by co-transfection with siLINC00313 and anti-NC, and defective miR-342-3p restoration was obtained by incubation of siLINC00313 and anti-miR-342-3p. (A) Expression levels of miR-342-3p were detected by qPCR. (B) Cell proliferation was assessed by MTT staining. (C) Cell apoptosis was detected with flow cytometry. (D and E) Activity of caspase 3 was assessed using Western blotting. (F and G) Cell migration and invasion were determined using transwell assay. (H) Positive cells of GFP-LC3 were calculated after transfection. (I) Expressions of Beclin 1, LC3-I, and LC3-II were detected with Western blotting. GAPDH was the loading control. All experiments were carried out in triplicate. *p<0.05.

Fig. 5