Figure 4.
qPCR results for odontoblast differentiation of DPSCs on resins with or without metformin (mean ± sd; n = 6). The gene expressions of DSPP (A), DMP-1 (B), ALP (C) and Runx2 (D) were analyzed at 1, 7 and 14 days. Compared to control resin, there was significant up-regulation of odontoblast-related genes: DSPP (A) at day 7 and day 14, and DMP-1(B) at day 1 and day 7. Mineral-related gene expression of ALP (C) at 7 and 14 days were also higher than that of DPSCs on control resin (p < 0.05). In each plot, values with dissimilar letters are significantly different from each other (p < 0.05).