FIGURE 4.

ROCK1 inhibitor Y‐27632 reverses PTEN activation, PI3K/Akt de‐phosphorylation and VEGF down‐regulation mediated by arctiin. (A‐H) HRCECs were pretreated with Y‐27632 (20 μmol/L) for 2 h, followed by treatment with arctiin for 48 h. Western blot was used to assess the expression of ROCK1, PTEN, PI3K, p‐PI3K, Akt and p‐Akt. β‐actin served as the loading control, and the relative quantification of proteins was analysed by Adobe Photoshop software.. (I) HRCECs were pretreated with Y‐27632 (20 μmol/L) for 2 h, followed by treatment with arctiin for 48 h. The cells supernatant were characterized by ELISA Kit assay. (J‐L) HRCECs were treated in (A), and Western blot was used to assess the expression of VEGFR2 and p‐VEGFR2. β‐actin served as the loading control, and the relative quantification of proteins was analysed by Adobe Photoshop software. All data are presented as the mean ± SD (*P < .05, ^** P < .01, ^*** P < .001)