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. 2020 Apr 16;24(10):5695–5706. doi: 10.1111/jcmm.15232

FIGURE 5.

FIGURE 5

ROCK1 inhibitor Y‐27632 abrogates arctiin‐disrupted cell cycle arrest in HG‐induced HRCECs. (A‐B) HRCECs were pretreated with Y‐27632 (20 µmol/L) for 2 h, followed by treatment with arctiin for 48 h. The cell cycle was analysed by flow cytometry, and the relative ratios of the G0/G1, S and G2/M phases were calculated using FlowJo software. (C‐F) Western blot analysis of cellular G0/G1 phase regulators CDK4, CDK2 and cyclin D1 after pretreatment with Y‐27632 (20 µmol/L) for 2 h, followed by treatment with arctiin for 48 h. β‐actin served as the loading control, and the relative quantification of proteins was analysed by Adobe Photoshop software. All data are presented as the mean ± SD (*P < .05, ** P < .01, *** P < .001)