FIGURE 2.
SENP3 conditional knockout (cKO) mice exhibited alleviated coagulation activation along with decreased tissue factor (TF) production in monocytes and alveolar macrophages during lipopolysaccharide (LPS)‐induced acute lung injury. SENP3fl/fl and SENP3 cKO mice (n = 6) were i.p. injected using LPS (30 mg/kg). A, The levels of thrombin‐antithrombin (TAT) complexes in the plasma were assessed by ELISA. B, The levels of TAT complexes in the bronchoalveolar lavage fluid (BALF) were assessed by ELISA. C, TF in CD11b (+) F4/80 (+) peripheral blood cells was assessed by flow cytometry. D, The statistics of the frequency of TF (+) CD11b (+) F4/80 (+) cells. E, Staining for TF and F4/80 was visualized in lung tissue sections via confocal microscopy, with representative images shown and TF staining intensity quantified. TF: green; F4/80: red; DAPI: blue. Scale bar, 10 or 100 μm. F, Quantification of TF fluorescence intensity is shown. Five F4/80 (+) cells were analysed per mouse (n = 6). Graphs show the mean ± SD, and data (A‐D, F) shown are representative of three independent experiments. *P < .05; **P < .01