Figure 6.

p‐STAT3 overexpression offsets the potentiation of Calcitriol on Doxorubicin‐induced apoptosis. A, The protein expressions of p‐STAT3 after p‐STAT3 expression lentivirus transduction in PTC cell lines. B, Cell viability of the PTC cell lines after p‐STAT3 expression lentivirus transduction. C, Flow cytometry analysis was used to detect the apoptosis in PTC cell lines after p‐STAT3 expression lentivirus transduction. D, Representative Western blotting for cleaved caspase‐3 and Bcl‐xl in PTC cell lines. E, The relative protein contents of cleaved caspase‐3 in PTC cell lines. F, The relative protein contents of Bcl‐xl in PTC cell lines. The protein expressions of nuclear PTPN2 were evaluated by Western blotting in PTC cell lines. Data were presented as the means ± SD and analysed by Student's t test or one‐way analysis of variance (ANOVA) followed by Tukey's multiple comparison test (n = 3), *P < .05 vs control or the indicated group