FIGURE 3.

ML264 inhibits invasion and migration in 143B and U2OS cells. A, 143B and U2OS cells were scratched using 200‐μL pipette tips, after which they were treated with different concentrations of ML264 for 24 h. B, The migration area was qualified. C, Cell migration assay was performed using a Transwell chambers for 24 h. D, The migration abilities of 143B and U2OS cells were quantified. E, Cell invasion assay was performed using Matrigel‐coated Transwell chambers for 24 h. F, The invasion abilities of 143B and U2OS cells were quantified. Data are expressed as the means ± SD of three independent experiments. *P < .05, **P < .01, ***P < .001 compared with the control group