Figure 4.

MSCs transplantation suppressed renal CD103⁺ DCs maturation in DN rat. A, Representative FACS analysis showing the gating strategy to identify number and maturation of CD103⁺ DCs in the kidneys. After pregating on CD45⁺ leukocytes, the CD11c⁺ CD103⁺ cells were gated, then the expression of costimulatory factors (CD80, CD86, markers associated with maturation of CD103⁺ DCs) on CD11c⁺ CD103⁺ DCs was analysed. B, Histogram of Representative FACS analysis showing expression of costimulatory factors (CD80, CD86) on CD103⁺ DCs in the kidney of NC, DN + Saline and DN + MSC rats after 12 weeks. Isotype controls (gray‐filled areas) and specific markers (red lines) are shown. C, Flow cytometric analysis of mean fluorescence intensity of costimulatory factors (CD80, CD86) on CD103⁺ DCs in the kidney of NC, DN + Saline and DN + MSC rats after 12 weeks. Data shown are the mean ± SD of evaluations from each group (n = 6 per group). ***P < .001 vs NC group, ###P < .001 vs DN + Saline group