Figure 7.

MSCs suppresses CD103⁺ DCs‐mediated CD8 T cells proliferation and activation. A, Representative FACS analysis showing the gating strategy to identify CD8⁺ T cells in vitro. Spleen mononuclear cells were incubated with CFSE and cocultured with or without CD103⁺ DCs (sorted form TNFα‐induced DCs) or/and MSC‐CM for 2 days. Then after pregating on CD8α⁺ T cells, CFSE intensity and the expression of Granzyme B in CD8α⁺ T cells was analysed. B and C, Percentage of CD8α⁺ T cells in the group of CD8α⁺ T cells cocultured with or without CD103⁺ DCs (sorted form TNFα‐induced DCs) or/and MSC‐CM for 2 days. Data shown represent the mean ± SD of each group (n = 5 per group). ***P < .001 vs NC group, ###P < .001 vs DN + Saline group. D, Representative FACS analysis showing expression of Granzyme B on CD8α⁺ T cells in the group of CD8α⁺ T cells cocultured with or without CD103⁺ DCs (sorted form TNFα‐induced DCs) or/and MSC‐CM for 2 days. E, Flow cytometric analysis of mean fluorescence intensity of Granzyme B on CD8α⁺ T cells in the group of CD8α⁺ T cells cocultured with or without CD103⁺ DCs (sorted form TNFα‐induced DCs) or/and MSC‐CM for 2 days. Data shown are the mean ± SD of each group (n = 5 per group). ***P < .001 vs NC group, ###P < .001 vs DN + Saline group. F, Real‐time PCR analysis of mRNA expression of TNFα and IFNγ in freshly isolated CD8α⁺ T from the group of CD8α⁺ T cells cocultured with or without CD103+ DCs (sorted form TNFα‐induced DCs) or/and MSC‐CM for 2 days. Data shown are the mean ± SD (n = 5 per group). ***P < .001 vs NC group, ##P < .01 vs DN + Saline group