FIGURE 2.

Down‐regulation of circPVT1 suppressed metastasis in HOS and 143B cells. A and B, CircPVT1 was knocked down by specific siRNAs sicircPVT1‐1 and sicircPVT1‐2 as confirmed by a qRT‐PCR assay. C and D, Transwell assays were performed after knocking down of circPVT1 in HOS and 143B cells. The migrated or invaded cells were counted in 10 randomly chosen microscopic fields (200×) of each experiment and pooled. E and F, Migration ability of HOS and 143B cells were checked by a wound healing assay. Data were shown as mean ± SD from three independent experiments. **P < .01 as normalizing and comparing with siSCR group