FIGURE 3.

CircPVT1 promoted metastasis via up‐regulation of FOXC2 in HOS and 143B cells. A, CircPVT1 was overexpressed and knocked down by transfection of oecircPVT1 and sicircPVT1 as measured by a qRT‐PCR, respectively. B, Expression of FOXC2 after up‐ and down‐regulation of circPVT1 was measured by a Western blot. C, A transwell migration assay was performed in HOS cells with three groups (vector, oecircPVT1 and oecircPVT1+siFOXC2), and in 143B cells with another three groups (siSCR, sicircPVT1 and sicircPVT1+oeFOXC2) D, A transwell invasion assay was also applied in HOS cells with three groups (siSCR, sicircPVT1 and sicircPVT1+oeFOXC2), and in 143B cells with another three groups (vector, oecircPVT1 and oecircPVT1+siFOXC2). The migrated or invaded cells were counted in 10 randomly chosen microscopic fields (100×) of each experiment and pooled. E and F, Migration ability of HOS (E) and 143B (F) cells was determined by a wound healing assay. Each sample was run in triplicate and in multiple experiments for mean ± SD. **P < .01 compared to controls, respectively