FIGURE 4.

CircPVT1 regulated FOXC2 partially via miR‐526b. A, Expression of FOXC2 mRNA after up‐ and down‐regulation of circPVT1 was evaluated by a qRT‐PCR assay. B, MiRNAs targeted circPVT1 and FOXC2 were predicted by using of circBank, circular RNA interactome and Targetscan. MiR‐526b and miR‐513a‐5p were overlapped in the aforementioned 3 databases. C, A diagram presented the seeding sequences of miR‐526b in circPVT1 and FOXC2. D, Expression of miR‐526b was significantly lower in OS than that of in normal bone according to an analysis of GEO database GSE28423. E and F, Expression of miR‐526 in collected 48 OS samples was measured by a qRT‐PCR assay. (g) Expression of miR‐526b at cellular level was determined by a qRT‐PCR assay. H, A Kaplan‐Meier analysis indicated that lower miR‐526b was correlated with shorter overall survival in 48 OS patients. I, Expression of miR‐526b after up‐ and down‐regulation of circPVT1 was determined by a qRT‐PCR assay, either. J, Correlation between circPVT1 and miR‐526b as measured by Pearson's correlation coefficient. K and L, Expression level of FOXC2 protein after cotransfection of oecircPVT1 and miR‐526b mimics (K) or sicircPVT1 and miR‐526b inhibitor (L) was measured by a Western blot. Each sample was run in triplicate and in multiple experiments for mean ± SD. ^n.s P > .05, **P < .01 and ***P < .001 compared to controls, individually