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. 2020 Apr 17;24(10):5565–5577. doi: 10.1111/jcmm.15213

Figure 3.

Figure 3

PRMT5 silencing by shRNA in MTAP‐negative (NCI‐H2452, MMB‐1 and IST‐MeS2) and MTAP‐positive (MPP 89) MM cells and in a non‐tumour mesothelial cell line (MeT‐5A). A, Western blotting analysis of PRMT5 in cells expressing shRNAs against PRMT5 (shPRMT5), in cells expressing a non‐targeting (NT) control shRNA and in non‐transduced parental cells (Par). An antibody against β‐actin was used as a loading control. B, Real‐time qRT‐PCR analysis of PRMT5 in the same cells as in (A). PRMT5 expression was calculated by the 2−ΔΔ C t method in shPRMT5 and NT‐shRNA expressing cells relatively to parental cells. Results are reported as means with standard deviations of three independent experiments. Statistical analysis was performed by subjecting the ΔC t values to one‐way repeated measures ANOVA with Tukey's post‐test. Statistically significant differences are indicated with: **very significant (P < .01) and ***: extremely significant (P < .001)