Figure 4.

PRMT5 silencing selectively affects the growth and clonogenic potential of MTAP‐deleted MM cells. A, Growth curves of non‐transduced parental cells (Par) and of cells expressing shRNAs against PRMT5 (shPRMT5) or a non‐targeting (NT) control shRNA. Cells were seeded at time‐point zero (T0) and counted at 24, 48 and 72 h after seeding. Results are reported as means with standard deviations of three independent experiments. PRMT5 knock‐down significantly impaired the growth of the MTAP‐negative MM cells, NCI‐H2452, IST‐MeS2 and MMB‐1 (statistical significance was determined by two‐way repeated measures ANOVA, P < .001), without affecting the growth of the MTAP‐positive MM cells, MPP 89, and non‐tumour mesothelial cells, Met‐5A. B, Representative dishes, out of three clonogenic assays, of parental MM cells and of MM cells expressing shPRMT5 and NT‐shRNA. The mean numbers of colonies with standard deviations obtained from the three experiments are reported in the histograms. Data were subjected to one‐way repeated measures ANOVA with Tukey's post‐test. PRMT5 silencing significantly affected the colony formation ability of NCI‐H2452 and MMB‐1 (***P < .001), without impairing the clonogenic potential of MPP 89 cells