Figure 5.

Effect of PRMT5 silencing on the E2F1 pathway in MM cells. A, The expression of E2F1 and of the E2F1‐regulated genes, cyclin A2 (CCNA2), cyclin E1 (CCNE1), enhancer of zeste homolog 2 (EZH2) and thymidylate synthase (TS), was evaluated by real‐time qRT‐PCR analysis in NCI‐H2452 cells expressing shPRMT5 or the NT control, relatively to non‐transduced parental cells. Relative expression was calculated by the 2^{−ΔΔ C t} method. Results are reported as means with standard deviations of three independent experiments. Statistical analysis was performed by subjecting the ΔC _t values to one‐way repeated measures ANOVA with Tukey's post‐test. Statistically significant differences are indicated with: *significant (P < .05), **very significant (P < .01) and ***: extremely significant (P < .001). B, Relative expression of the same E2F1‐regulated genes was also evaluated in the MTAP‐positive cells, MPP 89. No statistically significant differences were observed among parental cells, NT‐shRNA expressing cells and shPRMT5 expressing cells