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. 2020 Apr 12;24(10):5707–5717. doi: 10.1111/jcmm.15233

FIGURE 2.

FIGURE 2

HK2 expression mediated GBM cell proliferation and chemosensitivity. A, The growth of U87 (left) and A172 (right) with stable transfection of control or HK2 shRNA by lentivirus. B, The cell viability of U87 (left) and A172 (right) with stable transfection of control or HK2 shRNA upon different dosages of TMZ treatment for 24 h. C, The apoptosis of U87 (upper) and A172 (lower) with stable transfection of control or HK2 shRNA upon 10 µmol/L TMZ treatment for 24 h. D, The colony formation of U87 cells treated as in (C). E, The annexin‐V/PI staining of U87 cells treated as in (C) was analysed by flow cytometry. F, The expression of HK2 and cleaved caspase‐3 (cas‐3) in U87 cells treated as in (C). G, The cytochrome c in cytosolic fraction of U87 cells treated as in (C). H, The flow cytometry analysis of U87 cells treated as in (C) followed by mitotracker‐red staining. Data were represented in means ± SEM. *P < .05; **P < .01