FIGURE 5.

Mir‐125 is the target of HOTAIR in mediating HK2 expression. A, The expression of miR‐125 and miR‐143 in TMZ responsive or resistant GBM patients. B, The expression of miR‐125 and miR‐143 in U87 and A172 parental or TMZ resistant cells. C, The expression of miR‐125 in U87 and A172 transfected with control or HOTAIR shRNA lentivirus. D, The interaction of HOTAIR and miR‐125 interaction were analysed by biotin‐labelled miR‐125 pull‐down assay. E‐H, The U87 and A172 cells stably transfected with control or HOTAIR shRNA were transfected with miR‐125 antagomir. E, The HK2 mRNA level. F, The HK2 protein expression level. G, The cell growth of U87 cells. H, The apoptosis of U87 cells in each group treated with 10 µmol/L TMZ treatment for 24 h. Data were represented in means ± SEM. ^N P > .05; *P < .05; **P < .01