KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit monoclonal anti-Plin1 | Cell Signaling | Cat# 9349 |
| Rabbit polyclonal anti-UCP1 | Abcam | Cat# 10983 |
| Goat polyclonal anti-Plin1 | Novus | Cat# 100-60554 |
| Rabbit polyclonal anti-RFP | Rockland | Cat# 600-401-379 |
| Armenian hamster polyclonal anti-CD31 | Herzog 2013 | Abcam #119341 |
| Rabbit polyclonal anti-PDGFRα | Santa Cruz | Cat# 338 |
| Goat polyclonal anti-PDGFRα | R&D | Cat# AF 1062 |
| Rabbit polyclonal anti-PDGFRβ | Santa Cruz | Cat# 432 |
| Rat monoclonal anti-PDGFRβ | eBioscience | Cat# 14-1402-82 |
| Rabbit monoclonal anti-pY762 PDGFRα | Cell Signaling | Cat# 24188 |
| Rabbit monoclonal anti-α-Smooth Muscle Actin | Cell Signaling | Cat# 19245 |
| Donkey anti-rabbit HRP conjugate | Jackson ImmunoResearch | Cat# 711-035-152 |
| Donkey anti-rabbit AF647 conjugate | Jackson ImmunoResearch | Cat# 711-605-152 |
| Goat anti-Armenian hamster AF647 conjugate | Jackson ImmunoResearch | Cat# 127-605-099 |
| Donkey anti-Goat AF488 conjugate | Jackson ImmunoResearch | Cat# 705-545-003 |
| Donkey anti-Rabbit Cy3 conjugate | Jackson ImmunoResearch | Cat# 711-165-152 |
| Donkey anti-Rabbit AF488 conjugate | Jackson ImmunoResearch | Cat# 711-545-152 |
| Donkey anti-Rat AF488 conjugate | Jackson ImmunoResearch | Cat# 712-545-150 |
| AF 700 anti-mouse/rat CD29 | BioLegend | Cat# 102218 |
| APC anti-mouse CD34 | BioLegend | Cat# 119310 |
| BV 421 anti-mouse CD45.2 | BioLegend | Cat# 109831 |
| PerCP/Cy5.5 anti-mouse CD31 | BioLegend | Cat# 102420 |
| PE/Cy7 anti-mouse TER-119/Erythroid Cells | BioLegend | Cat# 116221 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Tamoxifen (Tmx) | Sigma Aldrich | Cat# T5648 |
| Crenolanib | Selleck Chemicals | Cat# S2730 |
| CL316,243 (CL) | Cayman Chemical | Cat# 17499 |
| Complete protease inhibitor cocktail | Sigma Aldrich | Cat# 11836170001 |
| Collagenase Type 1 | Gibco | Cat# 17100-017 |
| Collagenase Type 2 | Worthington | Cat# LS004176 |
| DMEM | Corning | Cat# 10-017-CV |
| Fetal Bovine Serum | Atlanta Biologicals | S11550 |
| L-glutamine | Gibco | 25030 |
| Pen/strep | Gibco | 18140 |
| Critical Commercial Assays | ||
| ECL Western blotting substrate | Pierce | Cat# 32106 |
| BCA assay | Pierce | Cat# 23225 |
| iTaq SYBR Green Supermix | Bio-Rad | Cat# 1725122 |
| Fluoro Gel with DABCO | Electron Microscopy Sciences | Cat# 17985-02 |
| Zombie Green | BioLegend | Cat# 423111 |
| DAPI | Sigma Aldrich | Cat# D9542 |
| Masson’s Trichrome Stain Kit | Electron Microscopy Sciences | Cat# 26367-series |
| Deposited Data | ||
| Quantitative Datasets | This paper | doi:10.17632/26r263sm6w.1 |
| Experimental Models: Organisms/Strains | ||
| Mouse: Ubc-CreERtg | Ruzankina 2007 | JAX:007001 |
| Mouse: Sox2-Cretg | Hayashi 2002 | JAX:008454 |
| Mouse: PdgfraH2B:GFP | Hamilton 2003 | JAX:007669 |
| Mouse: R26LSL-fSf-tdTomato | Madisen 2015 | JAX:021875 |
| Mouse: Pdgfrafloxed | Tallquist & Soriano 2003 | JAX:006492 |
| Mouse: Pdgfrbfloxed | Schmahl 2008 | JAX:010977 |
| Mouse: PdgfraK.Flp | This paper | N/A |
| Mouse: PdgfraFlp | This paper | N/A |
| Mouse: PdgfrbFlp | This paper | N/A |
| Oligonucleotides | ||
| aex2: ACCTCCCACCAGGTCTTTCT | This paper | N/A |
| ain2: CTGTAAGCCCATCCCAGAGA | This paper | N/A |
| aex3: GCCAGCTCACTTCACTCTCC | This paper | N/A |
| bex2: GGGCTTCCAGGAGTGATACC | This paper | N/A |
| bin2: CCAGCTGGACTGAAGAGGAG | This paper | N/A |
| bex3: CCGAGCAGGTCAGAACAAAG | This paper | N/A |
| SAF: CAAACTCTTCGCGGTCTTTC | This paper | N/A |
| aRR: CCCCATAGCTCCTGAGACCT | This paper | N/A |
| FlpR: CTTGCACAGGATGTCGAACTG | This paper | N/A |
| Flp1: GGCAGTTCGTGGAGAGATT | This paper | N/A |
| Flp2: GCCTTCTGGGTCTTGTACTT | This paper | N/A |
| Recombinant DNA | ||
| pPGKPhiC31obpA | Raymond & Soriano 2007 | Addgene Plasmid #13795 |
| pFlpo | Raymond & Soriano 2007 | Addgene Plasmid #13792 |
| aR-K.Flp SA.lox.Neo/Stop.lox-PDGFRαK.T2A.Flp targeting vector for the mouse Pdgfra gene |
This paper | N/A |
| aR-Flp sa-lox-Pdgfra-lox-Flpo-attB-Neo-attP targeting vector for the mouse Pdgfra gene |
This paper | N/A |
| bR-Flp sa-lox-Pdgfrb-lox-Flpo-attB-Neo-attP targeting vector for the mouse Pdgfrb gene |
This paper | N/A |
| Lenti-mCMV/EF1alpha/HTLV-Cre-IRES-iRFP720-PGK-Puro | This paper | N/A |
| Lenti-mCMV/EF1alpha/HTLV-IRES-iRFP720-PGK-Puro | This paper | N/A |
| Software and Algorithms | ||
| ImageJ | NIH | https://imagej.nih.gov/ij/ |
| GraphPad Prism 7 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| BD FACS Diva | BD Biosciences | https://www.bdbiosciences.com/enus/instruments/research-instruments/research-software/flow-cytometry-acquisition/facsdiva-software |
| FlowJo | FlowJo | https://www.flowjo.com/ |
| NIS-Elements D3.2 | Nikon | https://www.microscope.healthcare.nikon.com/products/software/nis-elements |
| NIS-Elements AR | Nikon | https://www.microscope.healthcare.nikon.com/products/software/nis-elements |
| ZEN | ZEISS | https://www.zeiss.com/microscopy/us/products/microscope-software/zen.html |
| Other | ||
| LabDiet 5053 (normal chow) | Purina | LabDiet 5053 |
| High Fat Diet (HFD) | Research Diets | D12492 |