| Protein | Substitution | Mutagenesis primer (substituted nucleotides) |
|---|---|---|
| Dot1l | S1105D,P1106D | CTCAGCACGGGTGTGGACGACAAGCGCCGGGCTC |
| LSD1 | T60E | GGAGCAGCTGGAGAGCGAGAGCCTCGAAAGAAGGAGCC |
| G9a | S115A | AAGTCGTTCCCCTCTGCCCCCAGCAAGGG |
| G9a | S346A | AAATGGCGGAAAGACGCCCCGTGGGTGAAGCC |
| G9a | T853A | GGCATGGGATCTGGCCCCAGAGCGCTC |
| G9a | S115D | AAGTCGTTCCCCTCTGACCCCAGCAAGGGGGG |
| G9a | S346D | AAATGGCGGAAAGACGACCCGTGGGTGAAGCC |
| G9a | T853E | GACACGGCATGGGATCTGGAACCAGAGCGCTCTGA |
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