FIGURE 2.

Determination of the absence of pCM1_Cc and chpG in low‐virulence Clavibacter capsici variants. (a) PCR amplification of the plasmid‐backbone genes and putative virulence genes in two plasmids of low‐virulence C. capsici isolates. N, sterile water used as a negative control; M, 1 kb DNA size ladder. (b) Southern blot analysis of natural variants lacking pCM1_Cc, but carrying chpG, with chpG as a probe in the digoxygenin detection system. Genomic DNAs extracted from each isolate were digested with the restriction enzyme XhoI. (c) Geographical location of C. capsici natural isolates collected in Korea. The map was obtained from the National Geographic Information Institute in Korea