FIGURE 5.

Effect of chpG expression in low‐virulence Clavibacter capsici isolates. (a) Observation of symptom development on the infected leaves with pCM1_Cc‐lacking C. capsici isolates and strains complemented with chpG of PF008. All inoculated leaves were photographed at 7 days after inoculation. Mock, 10 mM MgCl₂; EV, empty vector‐GFP. (b) Quantification of the disease severity in pepper leaves infected with each strain. EV, empty vector‐GFP. M, 10 mM MgCl₂. The level of disease severity between PF008 and strains complemented with chpG of PF008 was analysed by a nonparametric Kruskal–Wallis test with Dunnett's multiple comparisons (p < .05), and the same letter indicates the similar level. (c). Western blot analysis of ChpG expression in ΔpCM1_Cc and isolate 1,106, using FLAG‐tag antibody (α‐FLAG). CBB, Coomassie brilliant blue staining