Fig. 2.

Sequence-based holistic breeding approach to accelerate genetic gains in chickpea breeding. Integration of functional omics, GWAS, parent selection, high-throughput sequencing and phenotyping, and genomic selection to improve and accelerate the development of superior chickpea varieties in breeding. Multi-omics data can enable positional cloning by providing information on genes/superior alleles in the target region. High-throughput sequencing and multi-location phenotyping will harness superior alleles with diverse genetic resources. Newly identified genes/superior alleles can feed directly into the breeding population for parental selection. Models can also be used to predict the genomic estimated breeding values (GEBVs) based on high-coverage sequencing and phenotyping data. The integration of sequencing-based approaches with breeding programmes will be reflected in high productivity gains under limited resources and time