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. 2020 May 11;40(5):BSR20192697. doi: 10.1042/BSR20192697

Figure 3. LINC00668 exerts positive influence on YY1 by sponging miR-532-5p.

Figure 3

(A) Qualified mRNA candidates were selected. (B,C) mRNA candidates’ expression were examined by qRT-PCR analysis after knockdown of LINC00668 and overexpression of miR-532-5p. (D,E) qRT-PCR analysis presented the YY1 expression in normal liver cell line/cancerous cell lines and peritumoral tissues and tumorous tissues. (F–I) RIP assay, pull-down assay and luciferase reporter assay were performed to verify the relationship among LINC00668, miR-532-5p and YY1. (H) The predicted binding sites between YY1-WT and miR-532-5p and the mutant sequence of YY1 were demonstrated. (J) The expression of YY1 and according protein level in HepG2 and SNU-387 cells after intervene of LINC00668, miR-532-5p and YY1 were detected by qRT-PCR. (K) The YY1 protein level was detected by Western blot. *P<0.05, **P<0.01, ***P<0.001.