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. 2020 May 11;11(5):345. doi: 10.1038/s41419-020-2501-5

Fig. 6. Inhibitory effects of OC2 knockdown on tumor growth and angiogenesis.

Fig. 6

a OC2 knockdown reduced the protein levels of the VEGFA/EGFL6 axis and the AKT/MAPK pathways in Caov3 and Skov3, followed by Western blotting assay. b CCK-8 assay showed that OC2 knockdown could inhibit Caov3 and Skov3 proliferation. c Representative images and quantitation of HUVECs tube formation. d Effect of OC2 knockdown on xenograft tumor growth from Skov3, the stripped tumors, tumor size and tumor weight as indicated (n = 4). e Representative images and quantitation of the microvessels and the OC2/VEGFA/EGFL6 axis in stripped tumors, followed by IHC assay. The tubes (c) and spots (e) were calculated by ImageJ software and statistically analyzed in five random fields; scale bars: 50 μm (c), 100 μm (e). Data are shown as mean ± SD of three independent experiments. *P < 0.05; **P < 0.01. (i) Caov3, (ii) Skov3.