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. 2020 May 11;11(5):355. doi: 10.1038/s41419-020-2481-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a IHC detection of p-S6 in renal cortex during postnatal development. Scale bar=20 μm. b Representative western immunoblot analysis of p-S6 in renal cortex. c Representative western immunoblot analysis of p-S6 in cultured podocyte during differentiation. d Western immunoblot analysis of p-S6 and PKM2 expression in Tsc1^fl/fl and Tsc1^−/−-podocytes. e Rapamycin caused a significant reduction in the protein levels of p-S6 and PKM2 in cultured podocyte. f Western immunoblot analysis of nephrin and synaptopodin with 10 nM rapamycin treatment for 14 days.