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. 2020 May 11;11(5):341. doi: 10.1038/s41419-020-2529-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Hierarchical clustering of TAGLN-overexpressing or control HCT116 cells based on differentially expressed mRNA levels. Each row represents one replica sample and each column represents an mRNA. Expression level of each gene in a single sample is depicted according to the color scale. b Pie chart illustrating the distribution of the top pathway designations for the upregulated genes in TAGLN-overexpressing HCT116 cells. c Hierarchical clustering of TAGLN-depleted (siTAGLN) and control (siScr) RKO cells based on differentially expressed mRNA levels. d Pie chart illustrating the distribution of the top pathway designations for the downregulated genes in siTAGLN transfected RKO cells. e qRT-PCR validation of selected genes from microarray, n = 2, *P < 0.05, **P < 0.005, ***P < 0.005. f Venn-diagram depicting the overlap between the upregulated genes in TAGLN-overexpressing HCT116 and the downregulated genes in TAGLN-depleted RKO cells.