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. 2020 May 11;11(5):356. doi: 10.1038/s41419-020-2514-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Schematic flowchart illustrated the pipelines for miRNA analyses. b RTCA proliferation analysis for SW480 cells transfected with miR-149-5p mimics. c The Ago2 RIP showed that Ago2 served as a platform for circ5615, miR-149-5p and miR-331-3p. d Schematic illustration showed the potential binding sites of miR-149-5p and miR-331-3p on circ5615. e Luciferase activity of circ5615 transfected with miR-149-5p or miR-331-3p mimics. Firefly luciferase activity was normalized by Renila luciferase activity. f Biotin-coupled CircRNA pull-down assays confirmed that miR-149-5p was effectively enriched by circ5615 in SW480 cells. g, h circ5615 was enriched with 3′-end biotinylated miR-149-5p in SW480 and HCT 116 cells. i The FISH assays showed that circ5615 and miR-149-5p were relatively co-localized in the cytoplasm of SW480 cells. j Luciferase reporter activity of circ5615 co-transfected with miR-149-5p mimics or mimics NC. Data are shown as mean ± SD (n = 3). Similar results were obtained in three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001, paired t-test.